Role of the XPC Gene Expression in the Prevention of Oxidative and DNA Damage to Lung Squamous Carcinoma Cells

Background and Hypothesis: Xeroderma pigmentosum group C (XPC) is a DNA repair protein involved in the detection of damage caused by oxidative lesions through global genomic repair. Carcinogens, chemotherapeutics, UV-lesions require XPC. Exposure to one environmental toxin, cigarette smoke (CS), leads cancer development can worsen outcomes those with cancer, however, precise mechanisms why susceptibility varies individually remains poorly understood. This study examines role XPC cell survival human lung squamous cells (H520), hypothesizes that protects against death after exposure extracts (CSE).
 Methods: Lentiviral vector transduction for knock-down (XPCKD) was completed H520 cells. Transfection efficiency measured green fluorescence determine multiplicity infection (MOI); puromycin resistance CCK. targeted at two sites (Mission shRNA 118 119, Sigma), gene determined qRT-PCR. Survival unmodified H520-XPCKD CSE CCK clonogenic assays.
 Results: decreased expression 68-78% as RT-qPCR, confirmed Western blot. There increased XPCKD compared non-transduced cells.
 Conclusion: due smoke. Future studies will be performed confirm degree protection mechanism protective effect. These findings could important discussion risk factors patients understand risks smoking help physicians patient specific susceptibility.